KMID : 0880220100480040467
|
|
Journal of Microbiology 2010 Volume.48 No. 4 p.467 ~ p.472
|
|
Isolation and Characterization of Lactic Acid Bacteria Strains with Ornithine Producing Capacity from Natural Sea Salt
|
|
Yu Jin-Ju
Oh Suk-Heung
|
|
Abstract
|
|
|
Two lactic acid bacteria (LAB) having ornithine-producing capacity were isolated from Korean natural sea salt. They were Gram-positive, short rod-type bacteria, and able to grow anaerobically with CO2 production. The isolates grew well on MRS broth at 30-37¡ÆC and a pH of 6.5-8.0. The optimum temperature and pH for growth are 37¡ÆC and pH 7.0. The isolates fermented D-ribose, D-galactose, D-lactose, D-maltose, Dcellobiose, D-tagatose, D-trehalose, sucrose, D-melezitose, gentiobiose, D-glucose but not D-melibiose, inositol, and L-sorbose. The 16S rDNA sequences of the two isolates showed 99.5% and 99.6% homology with the Weissella koreensis S5623 16S rDNA (Access no. AY035891). They were accordingly identified and named as Weissella koreensis MS1-3 and Weissella koreensis MS1-14, and produced intracellular ornithine at levels of 72 mg/100 g cell F.W. and 105 mg/100 g cell F.W. and extracellular ornithine at levels of 4.5 mg/100 ml and 4.6 mg/100 ml medium, respectively, by culturing in MRS broth supplemented with 1% arginine. High cell growth was maintained in MRS broth with a NaCl concentration of 0-6%. These results show for the first time that Korean natural sea salts contain lactic acid bacteria Weissella koreensis strains having ornithine producing capacity.
|
|
KEYWORD
|
|
natural sea salt, ornithine, lactic acid bacteria
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|